NGYX I.C. developped a very simple MS-EXCEL worksheet from which you can in a few minutes check out about characteristics of PCR (or Sequencing, or ...) primers.
IT'S FREE. Just download the MS-EXCEL workbook (click HERE) and test it.
Important: Our approach is based on > 25 years of expertise/experience in the design and use of functional primers ( > 10,000) over a broad range of Template Types, Experiments Specificities, Environments (QA/QC), etc. So we hopefully captured essentials to perform optimal design of primers. BUT nobody is perfect! So we would really appreciate to receive your input. By advance, many thanks!